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The upper aqueous phase containing RNA was carefully collected, and RNA was precipitated with 250 L of isopropanol

Alcohol is not strictly contraindicated with either compound, but it undermines the protocol in multiple ways

In this setting, oxidative stress acts primarily as a persistent, low-grade metabolic stressor, arising from mitochondrial dysfunction and NADPH oxidase activation, and amplified by inflammatory cytokines (TNF, IL-6)

Liposomal delivery systems encapsulate AHK-Cu in lipid spheres, potentially improving penetration through the stratum corneum and protecting the peptide from degradation