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Published: February 12, 2014 This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose

Proteins were transferred to a nitrocellulose membrane using a semidry transfer system (Bio-Rad) in transfer buffer (25 mM Tris, 150 mM glycine, and 20% MeOH) for 2 h at 15 V

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Recent studies with ASCT2 knockout cells reveal that several compensatory mechanisms compensate for its absence, and that it nonetheless plays a role in tumorigenesis in ways that remain to be elucidated

doi: 10.1097/FJC.0000000000000438