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Digestive symptoms are the most common entry point: bloating, abdominal discomfort, and unpredictable bowel habits that seem tied to food but are hard to pin down

The resulting nanoparticles were washed and concentrated by ultrafiltration (Amicon Ultra-4 centrifugal filter units, molecular weight cut-off 100 kDa) with 3000 g for 10 min to achieve a final concentration of 10 mg mL 1

Dcard BF, Von Ahsen N, Grunwald T, et al

Lentivirus Vector Infection Lentiviruses expressing FTO, SLC7A11, FTO-shRNA (target sequences: gcAGCATACAACGTAACTTTG), SLC7A11-shRNA (target sequence: ccCTGGAGTTATGCAGCTAAT), scrambled shRNA, and empty vector were purchased from Genechem Company (Shanghai, China)

As shown in Figure 2D,E, significantly increased number of apoptotic cells was found in the TBI group relative to the sham group