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96 The disruption of normal protein phosphorylation and dephosphorylation processes can have various cellular effects, including alterations in the cytoskeleton, cell cycle progression, and apoptosis

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in NP-MSCs, the combined effect of hypoxia and nutrient deprivation inhibits cell proliferation, induces apoptosis, enhances caspase-3 activity, and downregulates functional genes (e.g., proteoglycan, collagen-I and -II) and stem cell-related genes (including Oct4, Nanog, Jagged, and Notch1) (Tian et al., 2020)

The expression of specific mRNAs was assayed by fluorescence-based quantitative real-time PCR (RT-qPCR) using a Fast-SYBR PCR Master Mix (Applied Biosystems)