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Cells were washed to remove viral particles and transduced cells were selected in the presence of 1 g/ml puromycin (Sigma-Aldrich, P8833) for at least 96 h

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These reductions were accompanied by significant body weight loss, confirming a robust pharmacodynamic response

Circadian light entrainment in Drosophila is mediated by the light-sensitive photoreceptor cryptochrome (cry) ( Drosophila , cry responds to blue light by binding to tim and recruiting the E3 ubiquitin ligase jetlag (jet), marking tim for proteasomal degradation and thereby resetting the molecular clock (Stanewsky et al., 1998

Acta astronautica 77, 207213, 10.1016/j.actaastro.2012.04.002 (2012)