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For soluble proteins , the supernatant was incubated with NiNTA beads (Thermo Fisher Scientific) while rotating at 4 C for 1 h

The localization of 2C ATPase , another nonstructural protein on the replication complex, was determined in the same cell using 2C mouse monoclonal antibody and Alexfluor 555-conjugated secondary antibody (red color)

Second, ERKO/Wnt-1 mice, which have no functional ER-, develop estrogen-induced mammary tumors23-25

DeepDriver 35 used gene mutation types as features, constructed a K-nearest neighbor graph based on the Pearson correlation coefficient, and trained a convolutional neural network to identify cancer risk genes

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