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University students, especially those studying abroad, represent a population in transition, often exposed to new social environments, cultural norms, and stressors that may encourage experimentation with tobacco products and affect their coping mechanisms and emotional regulation 5

The framing of the circle emblem is split into two parts the thick right half is colored silver, while the thinner left uses orange or blue, depending on the type of the cigarettes

Paraffin blocks were sectioned onto SuperFrost Plus slides, dried overnight, and baked for 1 h at 60 C before immediately proceeding with the RNAScope Multiplex Fluorescent v2 assay according to the manufacturers instructions with the following modifications: Target retrieval was performed for 15 min in a boiling beaker, Protease III was used for 30 min pre-treatment at 40 C and hybridized slides were left overnight in 5 SSC before proceeding with the amplification and labeling steps

There are fewer studies in low-income countries, in particular in Africa and India 24 , where the prevalence of e-cigarette use in teenagers is underreported

The use of cigarettes has been a subject of debate and scrutiny worldwide