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After washing, cells were incubated with FITC-labeled goat anti-rabbit IgG (H + L) (Beyotime, Shanghai, China) and DAPI for nuclear staining

After use, close the bottle carefully and rinse the measuring cup with warm water and dry

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More recently a number of genome-wide studies, using chromatin immunoprecipitation (ChIP), have increased our knowledge of androgen-regulated genes and have demonstrated a significant variability in DNA response element architecture, with imperfect palindromic sequences and half-sites identified as potential receptor binding sites (30, 127-131)

19 Biopsies are not common in these cases but show marked inflammation and massive liver necrosis