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qPCR was performed on immunoprecipitated DNA using SYBR Green Supermix

Methylene blue (MB), also known as methylthioninium chloride, is a synthetic heterocyclic aromatic compound with the molecular formula CHClNS and a molecular weight of 319.85 g/mol (NIST Chemistry WebBook)

The resulting bacterial infections, which are caused by organisms that have substantial genetic and metabolic flexibility, can resist multiple classes of antibiotics and evade phagocytic clearance

, NLR family pyrin domain containing 3 (NLRP3)) that sense pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs), such as the accumulation of ROS or the efflux of potassium ions (K + )

Becker K, Savvides SN, Keese M