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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

Reading Question #1 What is the term used to describe the property of the genetic code where a single amino acid can be specified by multiple similar codons

Tissues were then put into 0.15 M sodium cacodylate pH 7.4 containing 2.5% glutaraldehyde and fixed for additional 20 min at 4 C

It is conceivable that reduced zinc accumulation in insulin granules due to specific ZnT8 deficiency of -cells does not lead to hyperinsulinemia because hepatic clearance of pancreatic hormone is increased (282)