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Pellets were thawed on ice and lysed in 2X lysis buffer (100 mM TrisHCl pH 7.5, 300 mM NaCl, 2 mM EDTA, 2% (v/v) IGEPAL CA-630, 1% sodium deoxycholate, 0.5 mM TCEP (Tris(2-carboxyethyl)phosphine hydrochloride) with EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich) for 20 min on ice

RNA-seq analysis further revealed that GAP could restore UVB induced changes on expression of genes associated with pigmentation, inflammation, and aging

Farhat Z, Browne RW, Bonner MR, Tian L, Deng F, Swanson M, et al

We proposed to allow ACOs who wish to voluntarily terminate receipt of advance investment payments to do so and remain in the Shared Savings Program

Yaden DB, Griffiths RR