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Serial protein extraction from cell culture Soluble protein was extracted using RIPA buffer (supplemented with protease inhibitor, phosphatase inhibitor, and 50 mM N-Ethylmalamide) during immunoprecipitation assays (taken as Input prior to immunoprecipitation) or from lysate in cortical neuron experiments

The positive control peptide 5 showed higher binding to TNBC cells compared to peptide 1 (1.31.8-fold), however, positive control peptide 4 did not show much improvement in binding based on the peptide array-whole cell binding assay

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