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Tumor-suppressor p53: implications for tumor development and prognosis

Topalova-Dimitrova A, Dimitrov IV, Nikolov R

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Proline and its derivate hydroxyproline, account for 21 % of the amino-acid residues of the fibrous protein collagen, essential to connective tissue

Thereafter, anhydrous sodium sulfate was added to remove moisture from the product which was then filtered and distilled under reduced pressure to remove ethyl acetate, followed by fractional distillation under reduced pressure (93-95 C/5 mmHg) to obtain 40.4 g (yield: 80 %) of ethyl 4-chloro-3-hydroxybutyrate