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Once AOD 9604 is in solution, the goal is to limit the stresses that push a clean, usable preparation toward degradation, contamination, or a visible change that makes the sample questionable

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Thereafter, immediately placed in brain slicer with ventral side up to dissect out the hippocampus as previously mentioned 60,63 by inserting the blade at into the slots of the brain slicer just above and below the hypothalamus, to cut the brain into three slices which were then shifted to a petri dish placed on ice, moistened with chilled saline (0.9% NaCl)

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