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Materials and Methods Cloning, Site-Directed Mutagenesis, Protein Expression, and Purification Sequences coding for PtGSTU19 (Potri.008G174900) and PtGSTU20 (Potri.008G175000) were amplified by PCR from poplar cDNA using specific primers (Supplementary Table S1) and cloned into pET-12a plasmid (Novagen) between Nde I and Bam HI restriction sites

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These women are more susceptible to insulin resistance [112, 113], an effect that is mitigated by oestrogen replacement therapy [114,115,116]

Researchers are thus focusing on developing inhibitors that target drug-resistant isoforms, enhancing the effectiveness of existing chemotherapeutics

Although, the exact cause of these diseases is not identified, emerging research has highlighted that oxidative stress plays a critical role in their pathogenesis [3, 25]