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Researchers initially focused on understanding the biological role of endogenous amylin and how it contributes to nutrient sensing and postprandial signaling

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Salman Khan (Department of Pharmacy, QAU, Islamabad) for all their help and guidance without which this study would not have been possible

Based on the observed experimental features (gel-filtration behavior, stability to heating at 100 C, and amino acid) the authors concluded that PPI-1 possesses little ordered structure (Nimmo and Cohen, 1978)

NEO and BIO were detected using a fluorescence detector with excitation wavelength of 350 nm and emission at 450 nm.The concentrations of the studied metabolites were determined based on the areas of the peaks using external calibration curves