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The beads were finally taken up in 100 l 25 mM ammonium bicarbonate (ABC) containing 1 g Trypsin (Protein: Trypsin ratio 30:1)

Cytotoxicity in mammalian cells Vero cells (African Green Monkey Kidney cells, ATCC CCL-81) were grown in Dulbeccos modified Eagles medium (DMEM) containing glucose, L-glutamine and sodium pyruvate and supplemented with 10% fetal bovine serum and 1X penicillinstreptomycin (Sigma) at 37 C in culture flasks (TPT-90025) until confluent growth was attained

Chem Res Toxicol 2019;32:146986

The traditional herbal medicine, HangAmDan-B (HAD-B), reduces LPS-induced NO and PGE2 production in macrophages by blocking ATF2 phosphorylation, consequently limiting tumor-promoting activity of TAMs in gastric and colorectal cancers (166)