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The reaction mixture was subjected to denaturation (95C for 30 s), annealing (57C for 30 s), and extension (72C for 1 m) for 35 cycles in a Mastercycler gradient (Eppendorf, Germany)

10.1007/978-0-387-35766-9 7 Bzducha-WrbelA.BaejakS.KieliszekM.PobiegaK.FalanaK.JanowiczM

The findings of this review highlight the complexity of male infertility and underscore the need for further research to develop effective therapeutic interventions and improve reproductive outcomes

J Agric Food Chem 70(20):62136223 Chen H et al (2022) Patulin disrupts SLC7A11-cystine-cysteine-GSH antioxidant system and promotes renal cell ferroptosis both in vitro and in vivo

But it depends on whats getting in the way