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Controlled fluorescent labelling of antibody The engineered antibody was diluted in PBS containing 2 mM CTAC, achieving a final concentration of ~0.3 mg ml 1 , and then 1455 l of the above antibody solution was aliquoted in two microcentrifuge tubes

The ability of SASP secreted by senescent cells to induce CD38 NADase activity and NAD + consumption in neighboring cells thus establishes a niche-specific paracrine network linking cellular senescence and NAD + decline 27