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Following several rounds of optimisation, we determined the optimal expression conditions for the xCT/4F2hc heterodimer using suspension HEK293 cells and dual affinity tags for selective capture of correctly folded protein (Supplementary Fig

In the case of this imaginary peptide, the LCA of the input peptide is Unicorn, however the MISPs yield LCAs matching to several other Mythical Equus

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