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Inappropriate iron supplementation or excessive intake of iron supplements often leads to ROS accumulation through the Fenton and Haber-Weiss reactions, triggering oxidative stress, LPO, damage to intestinal epithelial cells, and even cell death, which disrupts the intestinal mucosal barrier function

Staining intensity was graded as: 0 (negative), 1 (weak), 2 (moderate), or 3 (strong)

The downregulation of stress-associated pathways and the simultaneous upregulation of protein synthesis and metabolism-related pathways suggest that NO supplementation via GSNO enhances cellular resilience, promotes protein homeostasis, and may attenuate the malignant progression of NEPC through these mechanisms

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